BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies. Biodegradation of Polycyclic Aromatic Hydrocarbons.

The spectra in figure 4 reveal the presence of aromatic compounds in the crudes treated with bacterial strains. They arrived to the laboratory for further processing within the first 24 h after their collection. Aliphatic hydrocarbons decreased in comparison with those of the abiotic control regarding the non-resolved background cycloalkanes, resins and asphaltenes. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants.

Based on our results, however, together with the existing literature, it is possible to make some inferences. In general, biodegradation is expected to be more extensive in aliphatic hydrocarbons, which are far more amenable to this process than their aromatic counterparts [35, 47, 48].

No alkanes with backbones shorter than 12 carbon atoms were detected in these samples. Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel. The latter, in turn, are more susceptible to biodegradation than resins and asphaltenes [47, 48]. The organic phase of the samples was extracted with 45 mL of HPLC-grade dichloromethane three extractions with a volume of 15 mL each using the liquid-liquid method for 30 min in a separating funnel, filtering the obtained organic extract through anhydrous reagent grade sodium sulfate.

Description of toluene inhibition of methyl bromide biodegradation in seawater and isolation of a marine toluene oxidizer that degrades methyl bromide.

Many Bacillus species have been shown to be able to degrade hydrocarbons [], both in terrestrial [] and marine or aquatic environments []. This phenomenon was more pronounced for strains F10S and F9S, which did not exhibit statistically significant differences when compared in this regard, but did so when compared to strain F1FLC. All spectra were processed using the Omnic v5.

Culturing these strains in the presence bactreias hydrocarbons Mesa 30 crude favored the expression of enzyme systems involved in their degradation and the preferential isolation of hydrocarbon-tolerant clones.


At the end, 18 strains representing each unique combination of these parameters were chosen to be subjected to the selection process. A Mycobacterium strain with letroleo capacities for degradation of gasoline hydrocarbons. Effectiveness of the natural dwgradadoras flora, bioestimulation and bioaumentation on the bioremediation of a hydrocarbon contaminated Antartic soil.

F9S exhibited the best results regarding the degradation of asphaltenes. Prentice Hall, Iberia; Some species of degraradoras genus have been isolated from marine environments contaminated with hydrocarbons [5, 17, 28]. Mechanisms for solvent tolerance in bacteria. The degradation potential exhibited by these new isolates warrants further studies on their possible application to decontaminate coastal environments affected by oil spills.

Taxonomic and metabolic variety notwithstanding, individual species seldom have the complete enzymatic toolset required to completely degrade the main organic compounds contaminating the ecosystem at any given time, and thus biodegradation usually proceeds through the concerted action of mixed populations degradadora microbial consortia.

Field evaluations of marine oil spill bioremediation. Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. This decrease in isoprenoids is a telltale sign of effective biodegradation; whereas strain F1FLC exhibits values above those of the abiotic control. All three strains under examination produced a notable decrease in the concentration of asphaltenes, compared to the control.

Bioremediation potential of terrestrial fuel spills. Strain selection Petrolro 33 isolated strains were grouped according to culture, morphological, physiological and biochemical parameters.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil. Las potencialidades biodegradadoras degradadoas estos microorganismos en la limpieza de costas marinas han generado nuevos estudios. Another two strains belonged to the genera Acinetobacter and Marinomonasrespectively. These microorganisms can be found not only in soil and water samples, but in clinical specimens, occasionally. Kurthia is a genus of Gram-positive bacilli described as environmental bacteria, whereas Azotobacter is a typical inhabitant of water bodies and soils.

Online J Biol Sci. Bacterial community dynamics and hydrocarbon degradation during a field-scale evaluation of bioremediation on a mudflat beach contaminated with buried bqcterias. Recent studies on the degradation of hydrocarbons in water bodies have made reference to the former [26, 36, 37]; this genus has, bactsrias addition, been found bacteias not only in terrestrial and marine environments, but in sewage as well.

Published reports indicate that they can also degrade phenanthrene in soils [38] as well as anthracene, phenol [41] and methyl methylbromide in marine environments [40]. Some authors place some species of this genus into Alteromonas instead [17].

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Most research on bioremediation technology has focused therefore on aerobic heterotrophic degradadorax, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3]. Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth Petfoleo sediments.

No statistically significant differences were found for this parameter among the examined strains, although the increased proportion of carboxyl and hydroxyl groups demonstrates the presence of biological oxidation processes. Microbial degradation of hydrocarbons in the environment. Pure stocks were obtained from colonies isolated by streaking, verifying their homogeneity by Gram staining and through the examination of culture characteristics.

Microbiol Mol Biol Rev. However, direct experimental observation has revealed that low molecular weight aromatic compounds may start to be degraded much earlier, sooner, in fact, than many aliphatic molecules [51].

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Naphthalene degradation and incorporation of naphthalene-derived carbon into biomass by the thermophile Bacillus thermoleovorans. ABSTRACT The isolation of aerobic marine bacteria able to degrade hydrocarbons represents a promising alternative for the decontamination of oceanic and coastal environments.

Two genera of Gram-positive bacilli were identified Bacillus and Kurthiaas well as five genera of Gram-negative bacilli Alcaligenes, Acinetobacter, Marinomonas, Pseudomonas and Azotobacter. Universidad de La Habana; Anaerobic microorganisms, however, are less versatile regarding their growth substrate and often display increased sensitivity toward heavy metals, hence playing a smaller role petrolleo biodegradation [2].

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Strain isolation The primary selection process employed two different discrimination protocols, denominated here protocol A modified from [8] and B modified from [9]. Had hydrocarbon composition dsgradadoras determined at that point, we would have most likely found practically identical levels of asphaltenes and resins in crudes treated with the strains under examination and in their controls, significantly decreased levels of saturated hydrocarbons in the former, and only a small drop in the concentration of aromatic hydrocarbons, since the bulk of their degradation takes place after 21 days.

Strain F10S1 lowered the concentration of all fractions in comparison with the control.