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Sequencing end-labeled DNA with base-specific chemical cleavages. Heat shock factor is regulated differently in yeast and HeLa cells. The human beta-interferon gene enhancer is under negative control. Images in this article Image on p.
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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Structure of a chromosomal gene for human interferon beta. Delimitation and properties of DNA sequences required for the regulated expression iirf human interferon-beta gene. Biochemistry of interferons and their actions.
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This article has been cited by other articles in PMC. Affinity purification of sequence-specific DNA binding proteins.
Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. National Center for Biotechnology InformationU.
Human embryonic lung cells HEL-R66 are of monkey origin. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements. Regulation of cytokine gene expression. Open in a separate window.
BK viral enhancer element and a human cellular homolog. Interaction of positive and negative regulatory proteins in the galactose regulon of yeast.
Support Center Support Center. A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells.
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Author information Copyright and License information Disclaimer. Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. Regulation of gene expression by interferons: In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the transcription of IFN-beta and possibly other genes.
igf Short and long range activation by the SV40 enhancer. Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
Associated Data Supplementary Materials. Human beta-interferon gene expression is regulated by an inducible enhancer element. High efficiency DNA-mediated transformation of primate cells. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.
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Controlled transcription of a human alpha-interferon gene introduced into mouse L cells. Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter.